7WN7

Crystal structure of HearNPV P26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	C 1 2 1
Unit cell lengths	194.749, 69.236, 52.746
Unit cell angles	90.00, 102.18, 90.00

Refinement procedure

Resolution	28.740 - 1.900
R-factor	0.1849
Rwork	0.184
R-free	0.21060
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.072	0.046	0.339
Rmeas	0.081	0.052	0.375
Rpim	0.035	0.023	0.157
Total number of observations	253451
Number of reflections	52908	5485	5114
<I/σ(I)>	5.1
Completeness [%]	97.1	98.4	94.7
Redundancy	4.8	4.7	4.6
CC(1/2)		0.996	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.7	289	PEG MME 5000