7WM3
hnRNP A2/B1 RRMs in complex with single-stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.190, 101.620, 111.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.027 - 1.620 |
| R-factor | 0.1636 |
| Rwork | 0.163 |
| R-free | 0.18450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1q |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2148) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.100 | 1.660 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.090 | 1.558 |
| Number of reflections | 118490 | 8688 |
| <I/σ(I)> | 19.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM LiCl, 0.1M HEPEs pH 7.0 , 20 % (w/v) polyethylene glycol (PEG) 6000 |
