7WKP
ICP1 Csy4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03923 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 57.892, 57.892, 355.786 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.040 - 2.000 |
| R-factor | 0.2156 |
| Rwork | 0.214 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xli |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.524 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.040 | 2.067 |
| High resolution limit [Å] | 1.996 | 1.996 |
| Rmerge | 0.145 | 1.880 |
| Number of reflections | 25046 | 2101 |
| <I/σ(I)> | 26.314 | |
| Completeness [%] | 98.4 | |
| Redundancy | 25.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.4M Sodium thiocyanate, 0.1M Sodium acetate pH 4.0, 16% w/v PEG 4000 |
