7WKJ
A COVID-19 T-cell response detection method based on a newly identified human CD8+ T cell epitope from SARS-CoV-2-Hubei Province, 2021.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Collection date | 2021-10-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 43.984, 81.902, 116.839 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.560 - 1.500 |
| R-factor | 0.2199 |
| Rwork | 0.219 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x7q |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.875 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.12) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.074 | 1.156 |
| Number of reflections | 68099 | 68099 |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2.0 M Ammonium sulfate |
