7WKJ

A COVID-19 T-cell response detection method based on a newly identified human CD8+ T cell epitope from SARS-CoV-2-Hubei Province, 2021.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	291
Detector technology	PIXEL
Collection date	2021-10-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 2 21 21
Unit cell lengths	43.984, 81.902, 116.839
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.560 - 1.500
R-factor	0.2199
Rwork	0.219
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x7q
RMSD bond length	0.006
RMSD bond angle	0.875
Phasing software	CNS
Refinement software	PHENIX (1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.074	1.156
Number of reflections	68099	68099
<I/σ(I)>	1.7
Completeness [%]	100.0
Redundancy	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2.0 M Ammonium sulfate