7WJ2
Crystal structure of HLA-C*1402 complexed with 8-mer HIV gag peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-12-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.655, 76.885, 62.053 |
| Unit cell angles | 90.00, 119.54, 90.00 |
Refinement procedure
| Resolution | 38.440 - 1.280 |
| R-factor | 0.1568 |
| Rwork | 0.156 |
| R-free | 0.17810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w6a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.056 | 0.384 |
| Number of reflections | 96254 | 4687 |
| <I/σ(I)> | 36.6 | |
| Completeness [%] | 99.0 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 100 mM sodium acetate, 20 % PEG Smear Low |
