7WIS

Catalytic intermediate structure of N381A mutant of copper amine oxidase from Arthrobacter globiformis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-09-07
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	C 1 2 1
Unit cell lengths	191.934, 63.472, 158.100
Unit cell angles	90.00, 117.30, 90.00

Refinement procedure

Resolution	26.359 - 1.900
R-factor	0.1643
Rwork	0.163
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.007
RMSD bond angle	1.105
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	140.486	26.359	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge		0.095	0.461
Rmeas	0.151	0.113	0.538
Rpim	0.077	0.060	0.275
Total number of observations	492849	15059	69549
Number of reflections	130556	4210	18760
<I/σ(I)>	6.8	15.7	2.5
Completeness [%]	98.0	96.2	97.1
Redundancy	3.8	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6.8	289	25 mM HEPES 1.05 M potassium sodium tartrate