7WHH

Crystal structure of SARS-CoV-2 omicron RBD and human ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-25
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.07180
Spacegroup name	P 41 21 2
Unit cell lengths	104.706, 104.706, 227.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.540 - 2.600
R-factor	0.1936
Rwork	0.192
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.660
High resolution limit [Å]	2.600	2.600
Rmerge	0.260	2.450
Number of reflections	39525	2586
<I/σ(I)>	11.3
Completeness [%]	99.8
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M L-Proline, 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 3350