7WH0

structure of C elegans BCMO-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	73.204, 104.733, 76.438
Unit cell angles	90.00, 102.16, 90.00

Refinement procedure

Resolution	31.630 - 1.800
R-factor	0.1671
Rwork	0.166
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rse
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.066	0.621
Rpim	0.027	0.259
Number of reflections	102764	9990
<I/σ(I)>	29.1	3
Completeness [%]	98.2	96.6
Redundancy	6.7	6.5
CC(1/2)	0.997	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	289	ammonium sulfate, PEG 3350.