7WEG

Complex structure of PDZD7 and FCHSD2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.978460
Spacegroup name	P 1 21 1
Unit cell lengths	41.603, 62.918, 46.030
Unit cell angles	90.00, 95.19, 90.00

Refinement procedure

Resolution	19.680 - 2.000
R-factor	0.2136
Rwork	0.209
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vrf
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.680	19.680	2.060
High resolution limit [Å]	2.000	8.960	2.000
Rmerge	0.125	0.055	0.715
Rmeas	0.136	0.059	0.774
Total number of observations	106495
Number of reflections	15785	174	1120
<I/σ(I)>	10.01	26.95	3
Completeness [%]	98.6	89.7	95.5
Redundancy	6.747	6.489	6.727
CC(1/2)	0.997	0.997	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.05M Zinc acetate dehydrate, 20% w/v polyethylene glycol 3350