7WDM

Fungal immunomodulatory protein FIP-gmi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	I 41 2 2
Unit cell lengths	96.585, 96.585, 80.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.040 - 2.123
R-factor	0.2061
Rwork	0.205
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f3h
Data reduction software	HKL-2000 (7.21)
Data scaling software	HKL-2000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.040	2.199
High resolution limit [Å]	2.123	2.124
Rmerge	0.117	0.915
Number of reflections	10434	1006
<I/σ(I)>	34.41
Completeness [%]	94.5
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293.15	0.1 M Na3Citrate (pH 5.6) 0.5 M NH4H2PO4