7WDC
Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 63 |
| Unit cell lengths | 95.261, 95.261, 182.682 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.130 - 2.840 |
| R-factor | 0.2852 |
| Rwork | 0.283 |
| R-free | 0.32510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dyj |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.214 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 3.010 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.085 | 0.915 |
| Number of reflections | 43729 | 6952 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.4 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 313 | sodium/pottasium tartrae, sodium acetate, KCl |
