7WD4
Crystal structure of the Ilheus virus helicase: implications for enzyme function and drug design
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 289.15 |
| Detector technology | PIXEL |
| Collection date | 2020-11-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.213, 112.213, 76.459 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.510 - 1.750 |
| R-factor | 0.1587 |
| Rwork | 0.157 |
| R-free | 0.19810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wv9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.729 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.071 | 0.293 |
| Number of reflections | 49792 | 49652 |
| <I/σ(I)> | 43.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289.15 | 0.1 M tris hydrochloride, pH 8.5, 0.2 M ammonium sulfate |
