7WCV

Co-crystal structure of FTO bound to 6e

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9735
Spacegroup name	H 3
Unit cell lengths	141.759, 141.759, 83.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.817 - 2.300
R-factor	0.2043
Rwork	0.203
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kqn
RMSD bond length	0.004
RMSD bond angle	0.646
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.820	28.820
High resolution limit [Å]	2.300	2.300
Rmerge	0.076	0.650
Number of reflections	27777	27777
<I/σ(I)>	31.2
Completeness [%]	99.6
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	100 mM sodium citrate, pH 5.6, 11.5% (w/v) polyethylene glycol (PEG) 3350,8% isopropanol.