7WBQ
Crystal structure of the receptor binding domain of SARS-CoV-2 Delta variant spike glycoprotein in complex with its receptor human ACE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-24 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.773, 143.654, 106.414 |
| Unit cell angles | 90.00, 95.29, 90.00 |
Refinement procedure
| Resolution | 44.700 - 3.340 |
| R-factor | 0.1989 |
| Rwork | 0.197 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lzg |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.629 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc3_4028) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.470 |
| High resolution limit [Å] | 3.340 | 3.340 |
| Rmerge | 0.162 | 1.307 |
| Number of reflections | 38716 | 3873 |
| <I/σ(I)> | 9.4 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.2 |
| CC(1/2) | 0.992 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M MES pH 6.5,10%w/v PEG 5000 MME,12% v/v 1-propanol |
