7WBG
BF2*1901/RY8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.127, 85.025, 110.838 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.400 - 2.000 |
| R-factor | 0.1856 |
| Rwork | 0.183 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yez |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.906 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (1.13_2998) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.142 |
| Number of reflections | 49775 | 4913 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 98.2 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.15 M Potassium bromide and 30% w/v Polyethylene glycol monomethyl ether 2,000 |
