7WAP
Mevo lectin mutant D134A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9799 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.810, 169.260, 160.940 |
| Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
| Resolution | 84.620 - 3.100 |
| Rwork | 0.228 |
| R-free | 0.27480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7bsb |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.989 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.630 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.148 | |
| Number of reflections | 118097 | 14028 |
| <I/σ(I)> | 4.7 | 2.1 |
| Completeness [%] | 91.8 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.970 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.6 | 295 | 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 20% v/v 2-Propanol, 20% w/v Polyethylene glycol 4000 |
