7WAI

Glutamyl-tRNA synthetase from Plasmodium falciparum (PfERS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	157.460, 47.363, 94.449
Unit cell angles	90.00, 109.10, 90.00

Refinement procedure

Resolution	45.373 - 2.105
R-factor	0.1966
Rwork	0.194
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nyl
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.15rc1_3423)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.400	2.142
High resolution limit [Å]	2.105	2.105
Rmeas	0.059	2.020
Number of reflections	35766	1100
<I/σ(I)>	15	0.6
Completeness [%]	92.6
Redundancy	6.3
CC(1/2)	1.000	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 30 % w/v PEG 4000