7WA3

Structure of American mink ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	67.697, 97.725, 105.063
Unit cell angles	90.00, 91.99, 90.00

Refinement procedure

Resolution	42.190 - 2.280
R-factor	0.2283
Rwork	0.228
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r42
RMSD bond length	0.003
RMSD bond angle	0.568
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.540	2.400
High resolution limit [Å]	2.280	2.280
Rmerge	0.142	0.591
Number of reflections	61639	9035
<I/σ(I)>	8.6
Completeness [%]	98.2
Redundancy	5.7
CC(1/2)	0.992	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Potassium thiocyanate, 30% w/v PEG 2000 MME2