7W9S
Crystal structure of the enterovirus 71 polymerase elongation complex (C1S3 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.992, 77.663, 150.831 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.935 - 2.530 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.24120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f8g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.620 |
| High resolution limit [Å] | 2.530 | 5.450 | 2.530 |
| Rmerge | 0.063 | 0.042 | 0.774 |
| Rmeas | 0.072 | 0.048 | 0.886 |
| Rpim | 0.033 | 0.022 | 0.418 |
| Total number of observations | 111816 | ||
| Number of reflections | 25127 | 2612 | 2335 |
| <I/σ(I)> | 20.2 | ||
| Completeness [%] | 95.2 | 92 | 90.6 |
| Redundancy | 4.5 | 4.2 | 3.6 |
| CC(1/2) | 0.997 | 0.645 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 289 | 0.085 M MES pH 6.5, 25.5 % (w/v) PEG 5000 monomethyl ether, 15 %(v/v) Glycerol |
