7W8I

Crystal structure of Zn bound human Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 21 21 21
Unit cell lengths	48.906, 53.099, 54.085
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.893 - 1.943
Rwork	0.226
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ow6
RMSD bond length	0.007
RMSD bond angle	1.585
Data reduction software	XDS (Version Jan 31, 2020)
Data scaling software	Aimless (0.7.4)
Phasing software	MoRDa (1.4.01)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.910	1.990
High resolution limit [Å]	1.940	1.940
Rmerge	0.138	4.590
Number of reflections	10806	646
<I/σ(I)>	12.4
Completeness [%]	99.4
Redundancy	12.8
CC(1/2)	0.998	0.325

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.05 M Zinc acetate dihydrate, 20% w/v Polyethylene glycol 3,350