7W8E
Sweet taste protein Brazzein - R43A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97933 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 51.634, 51.634, 60.618 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.410 - 1.340 |
R-factor | 0.2102 |
Rwork | 0.209 |
R-free | 0.22530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4heq |
RMSD bond length | 0.006 |
RMSD bond angle | 0.881 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.420 | 1.360 |
High resolution limit [Å] | 1.340 | 1.343 |
Rmerge | 0.115 | |
Rmeas | 0.119 | 0.124 |
Rpim | 0.039 | 0.055 |
Number of reflections | 20107 | 20107 |
<I/σ(I)> | 23.2 | |
Completeness [%] | 94.0 | |
Redundancy | 9.9 | |
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 1.5M NaCl, 1M Na-Citrate pH4.0 |