7W8D

The structure of Deinococcus radiodurans RuvC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	CCD
Collection date	2021-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	41.200, 72.770, 112.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.706 - 2.750
R-factor	0.275071348481
Rwork	0.273
R-free	0.30581
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ep4
RMSD bond length	0.004
RMSD bond angle	0.757
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.710	2.790
High resolution limit [Å]	2.750	2.750
Rmerge	0.131	0.722
Number of reflections	9594	674
<I/σ(I)>	13.4
Completeness [%]	91.2
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	289	0.2 M MgCl2, 0.1M HEPES (pH 7.8), 20% PEG 3350