7W86
Crystal structure of the DYW domain of DYW1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.28242 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.945, 45.165, 37.023 |
| Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
| Resolution | 36.660 - 1.800 |
| R-factor | 0.18029 |
| Rwork | 0.177 |
| R-free | 0.23365 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.524 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.170 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 10107 | 616 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.998 | 0.625 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Hepes pH 7.5, 0.1 M Sodium Acetate, 0.2 M Lithium sulfate, 25%(w/v) PEG4000 |
