7W82

Crystal structure of maize RDR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	H 3 2
Unit cell lengths	149.036, 149.036, 345.473
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.023 - 3.100
R-factor	0.232
Rwork	0.230
R-free	0.27030
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.291
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Rmerge	0.124	0.036	0.894
Rmeas	0.141	0.041	1.011
Rpim	0.067	0.020	0.468
Total number of observations	120266
Number of reflections	27101	2831	2682
<I/σ(I)>	3.7
Completeness [%]	99.7	98.7	99.9
Redundancy	4.4	4.2	4.6
CC(1/2)		0.998	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.6M Sodium Citrate, 0.1M MES, pH 6.5