7W74

Crystal structure of DTG rhodopsin from Pseudomonas putida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-20
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.080
Spacegroup name	P 63
Unit cell lengths	65.565, 65.565, 219.363
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.850 - 2.840
R-factor	0.2321
Rwork	0.229
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kfq
RMSD bond length	0.003
RMSD bond angle	0.575
Data reduction software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.850	44.850	3.010
High resolution limit [Å]	2.840	8.390	2.840
Rmerge	0.239	0.058	1.113
Rmeas	0.251	0.057	1.119
Number of reflections	12567	501	2007
<I/σ(I)>	8.81	28.11	2.01
Completeness [%]	99.9	98.4	100
Redundancy	10.7	10.9	9.8
CC(1/2)	0.997	0.999	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4	296	Magnesium chloride, Sodium chloride, Sodium citrate, PEG 300