7W70

Crystal structure of the PDZ-C domain fragment of Kangiella koreensis RseP orthologue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9800
Spacegroup name	P 21 21 21
Unit cell lengths	40.673, 42.408, 97.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.290 - 1.150
R-factor	0.1967
Rwork	0.196
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zpm
RMSD bond length	0.006
RMSD bond angle	0.822
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.410	1.170
High resolution limit [Å]	1.150	1.150
Rpim	0.017	0.350
Number of reflections	60798	2913
<I/σ(I)>	17.1	2.2
Completeness [%]	99.6
Redundancy	6.4
CC(1/2)	0.999	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.0 M ammonium sulfate, 0.1 M HEPES-Na (pH 7.5)