7W5H
The structure of trichobrasilenol synthase TaTC6 in complex with FsPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2021-06-17 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.34318 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.559, 98.567, 72.355 |
| Unit cell angles | 90.00, 94.59, 90.00 |
Refinement procedure
| Resolution | 31.230 - 2.050 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4okm |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 2.080 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.127 | 0.341 |
| Number of reflections | 91204 | 2000 |
| <I/σ(I)> | 7.1 | 2.4 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 4.1 | 3 |
| CC(1/2) | 1.000 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 297 | 1.2 M Sodium malonate, pH 6.0 |
