7W58

Crystal structure of acyl-carrier protein synthase from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-09-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5417
Spacegroup name	H 3
Unit cell lengths	67.640, 67.640, 85.779
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.630 - 2.270
R-factor	0.1691
Rwork	0.167
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gwm
RMSD bond length	0.004
RMSD bond angle	1.125
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	6746	180
<I/σ(I)>	21.44
Completeness [%]	97.5
Redundancy	8.3
CC(1/2)	0.990	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	Sodium acetate trihydrate, PEG 3350