7W43
Crystal structure of Bacillus subtilis YjoB N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.333, 84.176, 180.222 |
| Unit cell angles | 90.00, 112.29, 90.00 |
Refinement procedure
| Resolution | 49.360 - 3.000 |
| R-factor | 0.2393 |
| Rwork | 0.237 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7w42 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.121 | 0.710 |
| Number of reflections | 39002 | 1935 |
| <I/σ(I)> | 11.4 | 1.5 |
| Completeness [%] | 96.9 | |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 293 | 30% (w/v) isopropanol, 0.1 M Tris-HCl, 30% (w/v) PEG 3350 |
