7W2Q

Crystal structure of Pseudomonas aeruginosa transcriptional regulator PvrA in the narrow conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 41
Unit cell lengths	44.162, 44.162, 251.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.080 - 1.800
R-factor	0.1852
Rwork	0.184
R-free	0.21420
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	2.048
Data reduction software	HKL-3000
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.080	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	44339	4425
<I/σ(I)>	27.82
Completeness [%]	99.9
Redundancy	12.2
CC(1/2)	0.999	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292	0.1M MES,14% PEG 4000