7W2O

Crystal structure of Pseudomonas aeruginosa transcriptional regulator PvrA in the wide conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	84.098, 84.098, 148.378
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.030 - 3.210
R-factor	0.2724
Rwork	0.268
R-free	0.31360
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.871
Data reduction software	HKL-3000
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.030	3.300
High resolution limit [Å]	3.210	3.210
Number of reflections	9186	864
<I/σ(I)>	41.11
Completeness [%]	99.2
Redundancy	18.2
CC(1/2)	0.993	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.2M ammonium sulfate,0.1M sodium cacodylate,30%PEG8000