7W1H
glycosyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979150 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.959, 76.120, 63.518 |
Unit cell angles | 90.00, 97.04, 90.00 |
Refinement procedure
Resolution | 38.060 - 2.150 |
R-factor | 0.2009 |
Rwork | 0.199 |
R-free | 0.24760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l8z |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.550 | 2.220 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.146 | 0.819 |
Number of reflections | 26031 | 2289 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 99.0 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, SODIUM CHLORIDE, MES |