7W1G
Crystal structure of YfiH with C107A mutation in complex with UDP-MurNAc-L-Serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-11-08 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.556, 99.236, 136.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.450 - 1.860 |
| R-factor | 0.2175 |
| Rwork | 0.215 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f3v |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.562 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.940 |
| High resolution limit [Å] | 1.860 | 4.030 | 1.870 |
| Rmerge | 0.115 | 0.054 | 0.685 |
| Rmeas | 0.130 | 0.063 | 0.755 |
| Rpim | 0.059 | 0.032 | 0.311 |
| Number of reflections | 75568 | 6803 | 7199 |
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 92.7 | 79.6 | 89.3 |
| Redundancy | 5.1 | 3.8 | 5.6 |
| CC(1/2) | 0.993 | 0.852 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 2.0 M ammonium sulfate and 100 mM sodium acetate at pH 5.4 |
