7W15

Crystal Structure of MPH-E in complex with GTP and Erythromycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.973
Spacegroup name	P 1 21 1
Unit cell lengths	84.620, 50.278, 97.396
Unit cell angles	90.00, 115.41, 90.00

Refinement procedure

Resolution	34.850 - 1.770
R-factor	0.1881
Rwork	0.188
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.355
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.770	1.770
Rmeas	0.107	0.517
Number of reflections	72216	3600
<I/σ(I)>	19.7	3
Completeness [%]	99.7	96.5
Redundancy	6.7
CC(1/2)	0.988	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	O.O8 M Mggnesium acetate tetrahydrate; 0.05 M sodium cacodylate pH6.5; 25% PEG3350