7W09

UGT74AN2, Plant Steroid Glycosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979150
Spacegroup name	P 1 21 1
Unit cell lengths	54.413, 75.446, 61.766
Unit cell angles	90.00, 113.84, 90.00

Refinement procedure

Resolution	30.400 - 1.950
R-factor	0.2033
Rwork	0.202
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l8z
RMSD bond length	0.008
RMSD bond angle	0.896
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.770	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.107
Rmeas	0.126
Rpim	0.067
Number of reflections	32926	2268
<I/σ(I)>	10.3	2.9
Completeness [%]	98.8	97.4
Redundancy	6.7	6.3
CC(1/2)	0.996	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, sodium chloride, MES (pH 6.5)