7VY4

Snapshots of Human PSMD10(Gankyrin) unfolding by urea: 2 hours incubation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-03-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	60.300, 60.300, 122.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.220 - 2.220
R-factor	0.2475
Rwork	0.241
R-free	0.31210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uoh
RMSD bond length	0.007
RMSD bond angle	1.670
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.221	52.221	2.330
High resolution limit [Å]	2.220	7.000	2.224
Rmerge		0.033	1.702
Rmeas	0.295	0.035	1.813
Rpim	0.099	0.012	0.614
Number of reflections	13221	502	1688
<I/σ(I)>	7.5	20.5	0.5
Completeness [%]	98.2	99.8	88.1
Redundancy	8.6	7.7	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	60% Tacsimate pH7