7VXO

Structure of the C-terminal head domain of the Fowl Adenovirus serotype 4 (FAdV-4) fibre-2 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	293
Detector technology	CCD
Collection date	2021-06-26
Detector	ADSC QUANTUM 210r
Wavelength(s)	1
Spacegroup name	P 3 2 1
Unit cell lengths	60.303, 60.303, 92.095
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.240 - 1.600
R-factor	0.14
Rwork	0.138
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vtw
RMSD bond length	0.014
RMSD bond angle	1.752
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.103	0.296
Rmeas	0.106	0.060
Rpim	0.024	0.014
Total number of observations	475715
Number of reflections	25997	2808
<I/σ(I)>	6.7
Completeness [%]	98.6	86.5
Redundancy	18.3	14.2
CC(1/2)		0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Ammonium sulfate, 0.1M BIS-TRIS pH6.5, 25% w/v Polyethylene glycol 3350