7VWD
Crystal Structure of the Y53F/N55A mutant of LEH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-30 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.271, 50.391, 64.729 |
| Unit cell angles | 69.82, 79.87, 63.89 |
Refinement procedure
| Resolution | 26.000 - 2.153 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nww |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.478 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 26435 | 2620 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 93.1 | 92.58 |
| Redundancy | 4 | 4 |
| CC(1/2) | 0.989 | 0.952 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | NaCl, BIS-TRIS pH5.5, Polyethylene glycol 3350 |
