7VWB

Phloem lectin (PP2) structure -complex with N-Acetyllactosamine (LacNAc)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-02-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	37.690, 60.390, 82.980
Unit cell angles	90.00, 99.13, 90.00

Refinement procedure

Resolution	24.896 - 1.900
Rwork	0.218
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vs6
RMSD bond length	0.010
RMSD bond angle	1.567
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.190	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.130	0.180
Number of reflections	28551	4055
<I/σ(I)>	7
Completeness [%]	98.0
Redundancy	4
CC(1/2)	0.970	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	0.1 M MES monohydrate pH 6.0, 22% v/v Polyethylene glycol 400