7VW8

Helicoverpa armigera pheromone-binding protein PBP1 at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	32.030, 32.604, 54.792
Unit cell angles	90.00, 97.88, 90.00

Refinement procedure

Resolution	20.866 - 1.300
R-factor	0.1797
Rwork	0.179
R-free	0.19760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fjy
RMSD bond length	0.008
RMSD bond angle	1.378
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.066	0.500
Number of reflections	27772	1306
<I/σ(I)>	17.7
Completeness [%]	93.1
Redundancy	4.3
CC(1/2)	0.985	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	peg