7VVD

Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation Q135P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2018-09-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	40.505, 86.270, 126.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.793 - 3.134
R-factor	0.2464
Rwork	0.243
R-free	0.27430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v0c
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.200
High resolution limit [Å]	3.134	8.540	3.150
Rmerge	0.117	0.049	0.621
Rmeas	0.131	0.054	0.713
Rpim	0.058	0.023	0.342
Number of reflections	7537	462	355
<I/σ(I)>	5.9
Completeness [%]	93.3	98.1	96.7
Redundancy	4.9	5.2	4.4
CC(1/2)		0.997	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	25% PEG 1500, 0.1M MMT pH 8.0