7VUO

Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation F141L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2017-12-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 2 21
Unit cell lengths	41.623, 62.945, 82.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.719 - 2.679
R-factor	0.2444
Rwork	0.241
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v0c
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.740
High resolution limit [Å]	2.679	7.290	2.690
Rmerge	0.118	0.098	0.489
Rmeas	0.131	0.109	0.583
Rpim	0.056	0.047	0.307
Number of reflections	6398	381	303
<I/σ(I)>	12.7
Completeness [%]	98.3	99.2	91.3
Redundancy	4.9	5.2	3.1
CC(1/2)		0.965	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1M Tris8.5 25% w/v Polyethylene glycol 3,350 0.2M Trimethylamine N-oxide dihydrate