7VTR
3-ketoacyl-CoA thiolase Tfu_0875
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 154.470, 71.070, 158.648 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.680 - 1.930 |
R-factor | 0.1803 |
Rwork | 0.180 |
R-free | 0.21470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ulq |
Refinement software | PHENIX (v 1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Number of reflections | 131106 | 130937 |
<I/σ(I)> | 15 | |
Completeness [%] | 99.9 | |
Redundancy | 12.1 | |
CC(1/2) | 0.985 | 0.985 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289.15 | PEG 3350, Ammonium acetate, BIS-TRIS |