7VTM

Crystal structure of Glucoside hydrolase family 64 beta-1,3-glucanase complexed with Laminaritetraose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-01
Detector	DECTRIS EIGER2 X 1M
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	79.486, 79.486, 156.662
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.110 - 2.052
R-factor	0.21204109066
Rwork	0.211
R-free	0.22808
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vtk
RMSD bond length	0.014
RMSD bond angle	1.227
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2474)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.110	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.011
Rmeas		0.009
Number of reflections	36584	36584
<I/σ(I)>	31.4
Completeness [%]	97.0
Redundancy	19.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.1M sodium malonate pH 7.0, 0.1M HEPES pH 7.0