7VTJ
The cross-reaction complex structure with VQIIYK peptide and tau antibody's Fab domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-06-05 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.850, 70.380, 117.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.640 - 2.000 |
R-factor | 0.20322 |
Rwork | 0.196 |
R-free | 0.29470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lra |
RMSD bond length | 0.008 |
RMSD bond angle | 1.613 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.640 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.085 | 0.444 |
Number of reflections | 39350 | 39350 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 98.5 | |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 26% PEG3350, 20% Glycerol, 0.2M Ammonium iodide, 10mM Tris-HCl |