7VTI

Crystal structure of the Cas13bt3-crRNA binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9780
Spacegroup name	P 21 21 21
Unit cell lengths	78.434, 95.452, 125.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.990 - 1.890
R-factor	0.1971
Rwork	0.196
R-free	0.24290
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.797
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0291)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.990	48.990	2.000
High resolution limit [Å]	1.890	5.630	1.890
Rmerge	0.128	0.045	2.883
Rmeas	0.133	0.047	2.992
Total number of observations	2042011
Number of reflections	146058	5493	23484
<I/σ(I)>	14.59	55.89	0.93
Completeness [%]	99.9	99.8	99.3
Redundancy	13.981	13.489	13.886
CC(1/2)	0.999	0.999	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% PEG3350, 0.2 M Sodium bromide