7VT8

Crystal structure of MtGlu5 from Meiothermus taiwanensis WR-220

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-04-11
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	144.929, 144.929, 197.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.210 - 2.990
R-factor	0.2077
Rwork	0.205
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mmu
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.040
High resolution limit [Å]	2.990	2.990
Rmerge	0.071
Rmeas	0.076
Rpim	0.027	0.439
Number of reflections	21603	1055
<I/σ(I)>	26.55
Completeness [%]	99.8
Redundancy	7.8
CC(1/2)		0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		298	0.16M citric acid, 1.1M ammonium sulfate, 10% PEG-400, pH 5.0