7VT5

Crystal structure of CBM deleted MtGlu5 from Meiothermus taiwanensis WR-220

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-28
Detector	RAYONIX MX300HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	49.549, 75.348, 82.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.220 - 1.460
R-factor	0.1494
Rwork	0.148
R-free	0.17430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mmu
RMSD bond length	0.011
RMSD bond angle	1.145
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.510
High resolution limit [Å]	1.460	1.460
Rmerge	0.057	0.503
Rmeas		0.600
Rpim	0.034	0.320
Number of reflections	51808	4780
<I/σ(I)>	26.14
Completeness [%]	96.0
Redundancy	3.7
CC(1/2)		0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	20mM Tris, 100mM NaCl, pH 8.0