7VT4
Crystal structure of mutant E393Q of MtGlu5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-31 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.249, 114.350, 80.149 |
| Unit cell angles | 90.00, 103.23, 90.00 |
Refinement procedure
| Resolution | 26.970 - 1.930 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mmu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.949 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.930 |
| Rmerge | 0.059 | 0.059 |
| Rmeas | 0.068 | 0.872 |
| Rpim | 0.035 | 0.035 |
| Number of reflections | 97424 | 9740 |
| <I/σ(I)> | 22.06 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M citric acid, 0.7M ammonium sulfate, 20% PEG 400, pH 5.0 |
