7VSY

Pim1 with N82K mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-03-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	97.673, 97.673, 80.499
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.034 - 2.141
R-factor	0.1983
Rwork	0.196
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.158
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.220
High resolution limit [Å]	2.140	4.610	2.140
Rmerge	0.062	0.035	1.067
Rmeas	0.065	0.037	1.125
Rpim	0.020	0.011	0.356
Total number of observations	265143
Number of reflections	24119	2467	2403
<I/σ(I)>	14
Completeness [%]	100.0	99.6	100
Redundancy	11	10.6	9.9
CC(1/2)		0.999	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	277	Na.HEPES, NaCl